Not known Factual Statements About flow cytometers
Not known Factual Statements About flow cytometers
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Information-enriched characterization of cell populations. Morphological facts from photographs can insert to the richness of flow cytometry details. Such as, the figure exhibits an if not standard apoptosis assay utilizing Annexin V and PI, introducing mobile imaging to characterize cells in Each and every inhabitants to expose morphologically unique functions. These insights couldn't are already obtained from multiplex staining by itself.
Whether or not you’re a novice or a specialist, check out our methods to advance your investigate and knowledge in flow cytometry.
Combine acoustic concentrating engineering with large-parameter Investigation for adaptable spectral unmixing and standard workflows.
Find out new insights into your experimental data by visualizing populations employing merged fluorescence and morphology data. Our software package interface wizards permit downsampling, concatenation (merged information) and dimensionality instruments which are constructed to the application for an intuitive, streamlined information Examination encounter.
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On top of that, it can be illuminating to check out the quantity of doublets slip by means of traditional singlet gates, and terrific to verify the status of sudden double-optimistic occasions in my Evaluation."
Optimizing gating tactic. Even robust guide singlet gating is mistake-prone and continues to be a subjective final decision level in almost all flow cytometry assays. Imaging may be used to confirm and alter gates to include only solitary cells of interest.
Regular graphic high quality even at high throughput Acoustic concentrating in addition to a substantial-velocity digicam Blend to graphic these T cells constantly at reduced or superior flow rates. Very easily adjust aim and camera configurations to meet experimental specifications.
The Attune NxT cytometer would be the reputable workhorse instrument your lab can count on for reliable and reliable effectiveness. The Attune CytPix design includes a higher-pace brightfield digital camera and AI-pushed impression Investigation software, with current upgrades including device learning equipment and an intuitive person interface, enabling enhanced info precision, accuracy, and more rapidly insights into sample biology.
Maybe most significantly, these situations include cells of Obviously diverse phenotypes which may lead to incorrect conclusions relating to double favourable events (especially in scarce populations).
Seem waves help exact mobile alignment, causing Extraordinary details quality and ~10X more rapidly sample flow fees.
We Earlier demonstrated the power of imaging Car or truck-T/Ramos mobile interactions. Let’s have a look at just the population of greatest interest, the double good events, to learn more. We can now use extended morphological parameters (in this article, circularity and skewness of intensity) to additional analyze the features of these populations and refine gating on these activities, increasing details robustness.
Crafted-in dimensionality reduction instruments elevated resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to confirm expected population resolution in lysed full human blood. Consumer can watch events from Each and every cluster for Visible confirmation of cell phenotypes.
Adaptable payment approach from Agilent Money Methods let you obtain the critical instrumentation you would like for speedy venture, or to mature your lab.
At the center of every Attune Flow Cytometer lies our innovative acoustic concentrating engineering, which makes use of audio waves click here to meticulously align cells inside the flow cell. This innovative strategy surpasses regular hydrodynamic methods, causing a lot quicker processing speeds, Excellent details excellent, and enhanced sample throughput.
The Attune Xenith cytometer is a large-parameter spectral analyzer, supplying equally spectral unmixing and standard flow abilities for complete and versatile cell Assessment.
Agilent Value Promise ensures you no less than ten years of instrument use through the day you buy. Normally, We are going to credit you With all the residual price of the procedure towards an upgraded product.
What appeared to be a homogeneous population is definitely additional varied—an Perception that needs to be deemed when interpreting outcomes.